Predicting Hyperoxia-Induced Lung Injury from Associated Intestinal and Lung Dysbiosis in Neonatal Mice

<b><i>Background:</i></b> Preclinical studies have demonstrated that hyperoxia disrupts the intestinal barrier, changes bacterial composition, and injures lungs of newborn animals. <b><i>Objectives:</i></b> The aim study was to investigate effects on lung microbiota communication between develop a predictive model for identification hyperoxia-induced injury from based machine learning algorithms in neonatal mice. <b><i>Methods:</i></b> Neonatal C57BL/6N mice were reared either room air or (85% O<sub>2</sub>) postnatal days 1–7. On day 7, sampled left lower gastrointestinal tract 16S ribosomal RNA gene sequencing. Tissue right terminal ileum harvested Western blot histology analysis. <b><i>Results:</i></b> Hyperoxia induced injury, decreased tight junction expression, impaired alveolarization angiogenesis also altered promoted translocation intestine as evidenced by presence bacteria hyperoxia-exposed relative abundance these taxa significantly positively correlated with increased cytokines. <b><i>Conclusions:</i></b> dysbiosis lung. Further are needed clarify pathophysiology